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The titanium discs were either air-dried, soaked in cigarette smoke for 10 min, or subjected to artificial saliva solution (control) before implantation into the femoral condyle of rabbits. Six months later, the femora were harvested and examined with a scanning electron microscope (SEM). The surface characteristics of titanium discs before and after treatment were assessed with a contact angle meter. In addition, an osteoblast-titanium interaction assay was conducted on the discs. The results showed that the contact angle increased when the titanium discs were immersed in the cigarette smoke solution or artificial saliva solution. Interestingly, the contact angle of the titanium discs after air-drying was lower than the contact angle of the titanium discs after soaking in the cigarette smoke solution or artificial saliva solution. Osteoblast-titanium interaction analysis showed that the number of adherent osteoblasts and the average area of the osteoblasts on the titanium discs after air-drying were increased. However, the number of adherent osteoblasts and the average area of the osteoblasts on the titanium discs after soaking in the cigarette smoke solution were decreased compared with the titanium discs after air-drying. The average area of the osteoblasts on the titanium discs after soaking in the artificial saliva solution was increased compared with the titanium discs after air-drying but decreased compared with the titanium discs after soaking in the cigarette smoke solution. Conclusions {#s0130} =========== Our study showed that the surface characteristics of titanium changed after being exposed to cigarette smoke. There was no difference in the titanium surface characteristics after being exposed to the cigarette smoke solution and artificial saliva solution. However, the contact angle of the titanium discs after soaking in the cigarette smoke solution was lower than that after soaking in the artificial saliva solution. Significance {#s0135} ============ This study provides more comprehensive knowledge about the changes in the surface characteristics and osteoblast-titanium interactions when the titanium implants are subjected to cigarette smoke. This study was supported by the National Natural Science Foundation of China (Nos. 81772353 and 81772355), the Science and Technology Project of Hubei Health and Family Planning Commission (No. WJ2017F058), the Research Project of Wuhan Health and Family Planning Commission (No. WZ0999), and the Natural Science Foundation of Hubei Province (No. 2017CFB221). Disclosures: The authors declare

 

 


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X-FORCE Keygen Smoke 2019

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